Part:BBa_K1634005
pmyo3 (a promoter in C.elegans)
“myo-3 encodes MHC A, the minor isoform of MHC (myosin heavy chain) that is essential for thick filament formation, and for viability, movement, and embryonic elongation; expressed in body muscle, the somatic sheath cell covering the hermaphrodite gonad, and also expressed in enteric muscle, vulval muscles of the hermaphrodite and the diagonal muscles of the male tail. “(from Wormatlas) Since we want to control the C.elegans' movement.We decided to use pmyo-3 (promoter of myo-3) to construct a plasmid which can drive the expression in the worm’s body muscle. When we express the channelrhodopsins in some specific cells in organisms and shed specific light on them, we can activate or supress the specific ion channel to change the activity of the cell. According to this thought in optogenetic, we linked the Pmyo3 with channelrhodopsin, then we use the light source assembled by ourselves to shed light of specific wave on the C.elegans, changing the condition of the muscle, thus changing the movement of the C.elegans.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 259
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